Department of Engineering Science

Dept.of Engineering Science, Bioscience and Technology Program aims at education and research, from basics to application, on physics, chemistry, and biology, namely the core subjects for the development of quantum devices, materials engineering, and biotechnology that are essential for today's highly-developed communication society.


Yutaka Kano, Ph.D
Title: Professor
Email: kano @
Phone: +81-424-43-5586
Office: East-6 Bldg. 907

Research Fields
Applied Physiology: muscle plasticity to exercise, microcirculation, oxygen exchange, cell membrane function.

Summary of Research Activities
The purpose of our research is to examine the mechanisms contributing to the skeletal muscle blood flow, oxygen exchange and cell membrane function on disease muscle conditions such as aging, inactivity and exercise-induced muscle damage. Recently, we have demonstrated that eccentric exercise results in an altered capillary geometry in muscle. To date, however, it is unknown whether microcirculatory function and the capacity to deliver and distribute O2 within the capillary bed is impaired following eccentric exercise. In the present, we study the cell membrane function and oxygen delivery/oxygen utilization sequel to eccentric exercise. The laboratory has a rodent chamber, eccentric exercise equipment, microscopy for bio-imaging, cryostat, phosphorescence quenching equipment and EMG device.



In press
Sex differences in mitochondrial Ca2+ handling in mouse fast-twitch skeletal muscle in vivo. Daiki Watanabe, Koji Hatakeyama, Ryo Ikegami, Hiroaki Eshima, Kazuyoshi Yagishita, David Poole, and Yutaka Kano. Journal of Applied Physiology.


Kitaoka Y, Watanabe D, Nonaka Y, Yagishita K, Kano Y, Hoshino D. Effects of clenbuterol administration on mitochondrial morphology and its regulatory proteins in rat skeletal muscle. Physiol Rep. 2019;7(19):e14266.

Shutaro Kodama, Manami Ohta, Kanami Ikeda, Yutaka Kano, Yoko Miyamoto, Wolfgang Osten, Mitsuo Takeda, and Eriko Watanabe. "Three-dimensional microscopic imaging through scattering media based on in-line phase-shift digital holography. Appl Opt. 2019;58(34):G345−G350.

Tabuchi A, Eshima H, Tanaka Y, Nogami S, Inoue N, Sudo M, Okada H, Poole DC, Kano Y. Regional differences in Ca2+ entry along the proximal-middle-distal muscle axis during eccentric contractions in rat skeletal muscle. J Appl Physiol (1985). 2019;127(3):828−837.

Hirai DM, Craig JC, Colburn TD, Eshima H, Kano Y, Musch TI, Poole DC.Skeletal muscle interstitial Po2 kinetics during recovery from contractions. J Appl Physiol (1985). 2019;127(4):930−939.

Ryo Ikegami, Hiroaki Eshima, Takuro Mashio, Tomosada Ishiguro, Daisuke Hoshino, David Poole, and Yutaka Kano. Accumulation of intramyocyte TRPV1-mediated calcium during heat stress is inhibited by concomitant muscle contractions. J Appl Physiol. 2019 Mar 1;126(3):691-698.

Hirai DM, Colburn TD, Craig JC, Hotta K, Kano Y, Musch TI, Poole DC. Skeletal muscle interstitial O2 pressures: bridging the gap between the capillary and myocyte. Microcirculation. Microcirculation. 2019;26(5):e12497.


Hiroaki Eshima, David Poole, and Yutaka Kano. Mitochondrial calcium regulation during and following contractions in skeletal muscle. Review. The Journal of Physical Fitness and Sports Medicine

Kazuki Hotta, Bradley Behnke, Kazuto Masamoto, Rie Shimotsu, Naoya Onodera, Akihiko Yamaguchi, David Poole, and Yutaka Kano. Microvascular Permeability of Skeletal Muscle After Eccentric Contraction-Induced Muscle Injury: In Vivo Imaging Using Two-Photon Laser Scanning Microscopy. J Appl Physiol (1985). 2018 Aug 1;125(2):369-380. doi: 10.1152/japplphysiol.00046.2018. Epub 2018 May 3.

Toshiaki Nakajima, Seiichiro Koide, Tomohiro Yasuda, Takaaki Hasegawa, Tatsuya Yamasoba, Syotaro Obi, Shigeru Toyoda, Fumitaka Nakamura, Teruo Inoue, David Poole, and Yutaka Kano. Muscle hypertrophy following blood flow-restricted low force isometric electrical stimulation in rat tibialis anterior: Role for muscle hypoxia. J Appl Physiol (1985). 2018 Jul 1;125(1):134-145. doi:10.1152/japplphysiol.00972.2017. Epub 2018 Mar 22.

Daniel M. Hirai, Jesse C. Craig, Trenton D. Colburn, Hiroaki Eshima, Yutaka Kano, William L. Sexton, Timothy I. Musch, and David C. Poole. Skeletal muscle microvascular and interstitial PO2 from rest to contractions. J Physiol. 2018 Mar 1;596(5):869-883. doi: 10.1113/JP275170. Epub 2018 Jan 30.


Aiko Watanabe, David C. Poole and Yutaka Kano. The effects of RSR13 on microvascular PO2 kinetics and muscle contractile performance in the rat arterial ligation model of peripheral arterial disease. J Appl Physiol (1985). 2017 Oct 1;123(4):764-772. doi: 10.1152/japplphysiol.00257.2017.

Hiroaki Eshima, Shinji Miura, Nanami Senoo, Koji Hatakeyama, David C. Poole and Yutaka Kano. Improved skeletal muscle Ca2+ regulation in vivo following contractions in mice overexpressing PGC-1α. Am J Physiol Regul Integr Comp Physiol. 2017 Jun 1;312(6):R1017-R1028. doi: 10.1152/ajpregu.00032.2017. Epub 2017 Apr 24.

Toru Tomimatsu, Jun Miyazaki, Yutaka Kano, and Takayoshi Kobayashi. Photothermal imaging of skeletal muscle mitochondria.Biomedical Optics Express Vol. 8, Issue 6, pp. 2965-2975 (2017)

Mario Wakizaka, Hiroaki Eshima, Yoshinori Tanaka, Hideki Shirakawa, David C. Poole and Yutaka Kano. In vivo Ca2+ dynamics induced by Ca2+ injection in individual rat skeletal muscle fibers. Physiol Rep. 2017 Mar;5(5). pii: e13180. doi: 10.14814/phy2.13180.

Sudo Mizuki, Ando Soichi, and Kano Y. Repeated blood flow restriction induces muscle fiber hypertrophy. Muscle Nerve. 2017 Feb;55(2):274-276. doi: 10.1002/mus.25415.


Yoshinori Tanaka, Tadakatsu Inagaki, DavidC Poole and Yutaka Kano. pH buffering of single rat skeletal muscle fibers in the in vivo environment. Am J Physiol Regul Integr Comp Physiol. 2016 May 15;310(10):R926-33. doi: 10.1152/ajpregu.00501.2015.
電気通信大学Research Highlightsとして紹介されています.

Yoshinori Tanaka, David C. Poole and Yutaka Kano. pH homeostasis in contracting and recovering skeletal muscle: Integrated function of the microcirculation with the interstitium and intramyocyte milieu. Curr Top Med Chem. 2016;16(24):2656-63. Review.

Toshiaki Nakajima, Tomohiro Yasuda, Seiichiro Koide, Tatsuya Yamasoba, Syotaro Obi, Shigeru Toyoda , Yoshiaki Sato, Teruo Inoue and Yutaka Kano. Repetitive restriction of muscle blood flow enhances mTOR signaling pathways in a rat model. Heart Vessels. 2016 Oct;31(10):1685-95. doi: 10.1007/s00380-016-0801-6.


Kohei Yamakoshi, Kazuyoshi Yagishita, Hirotsugu Tsuchimochi, Tadakatsu Inagaki Mikiyasu Shirai, David C Poole and Yutaka Kano Microvascular oxygen partial pressure during hyperbaric oxygen in diabetic rat skeletal muscle.
Am J Physiol Regul Integr Comp Physiol. 2015 Dec 15;309(12):R1512-20. doi: 10.1152/ajpregu.00380.2015. Epub 2015 Oct 14.

Sudo M, Ando S, Poole DC, Kano Y. Blood flow restriction prevents muscle damage but not protein synthesis signaling following eccentric contractions. Physiol Rep. 2015 Jul;3(7). pii: e12449. doi: 10.14814/phy2.12449.

Eshima H, Poole DC, Kano Y. In vivo Ca2+ buffering capacity and microvascular oxygen pressures following muscle contractions in diabetic rat skeletal muscles: fiber-type specific effects. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology. 2015 Jul 15;309(2):R128-37. doi: 10.1152/ajpregu.00044.2015. Epub 2015 May 6.


Eshima H, Poole DC, Kano Y. In vivo calcium regulation in diabetic skeletal muscle. Cell Calcium. 2014 Nov;56(5):381-9. doi: 10.1016/j.ceca.2014.08.008. Epub 2014 Aug 27.

Yutaka Kano, Shinji Miura, Hiroaki Eshima, Osamu Ezaki and David C. Poole. The effects of PGC-1α on control of microvascular PO2 kinetics following onset of muscle contractions. J Appl Physiol (1985). 2014 May 15. pii: jap.00080.2014. [Epub ahead of print]PMID: 24833782


Hiroaki Eshima, Yoshinori Tanaka, Takashi Sonobe, Tadakatsu Inagaki, Toshiaki Nakajima, David C. Poole, and Yutaka Kano: In vivo imaging of intracellular Ca2+ after muscle contractions and direct Ca2+ injection in rat skeletal muscle in diabetes. Am J Physiol Regul Integr Comp Physiol. 2013 Sep 15;305(6):R610-8. doi: 10.1152/ajpregu.00023.2013. Epub 2013 Jul 24.PMID: 23883681

Yutaka Kano and Kunihiro Sakuma: Effect of aging on the relationship between capillary supply and muscle fiber size. Adv. Aging Res. 2: 37-42, 2013.

Tomonobu Sakurai, Osamu Kashimura, Yutaka Kano, Hideki Ohno, Li Li Ji, Tetsuya Izawa, Thomas M. Best. Role of nitric oxide in muscle regeneration following eccentric muscle contractions in rat skeletal muscle. J Physiol Sci. 2013 Jul;63(4):263-70. doi: 10.1007/s12576-013-0262-y. Epub 2013 Apr 21.PMID: 23606218


Paul McDonough, Danielle J. Padilla, Yutaka Kano, Timothy I. Musch and David C. Poole, and Brad J. Behnkee. Plasticity of microvascular oxygenation control in rat fast-twitch muscle: Effects of experimental creatine depletion. Respir Physiol Neurobiol. 2012 Apr 15;181(1):14-20. doi: 10.1016/j.resp.2012.01.003. Epub 2012 Jan 18.PMID: 22285799

Shunsaku Koga, Yutaka Kano, Thomas Barstow, Leonardo Ferreira, Etsuko Ohmae, Mizuki Sudo, and David Poole: Kinetics of muscle deoxygenation and microvascular PO2 during contractions in rat: Comparison of optical spectroscopy and phosphorescence-quenching techniques. J Appl Physiol (1985). 2012 Jan;112(1):26-32. doi: 10.1152/japplphysiol.00925.2011. Epub 2011 Oct 6.PMID: 21979807

Yutaka Kano,Takashi Sonobe,Tadakatsu Inagaki, Mizuki Sudo and David C Poole. Mechanisms of exercise-induced muscle damage and fatigue: Intracellular calcium accumulation. J .Phys. Fitness Sports Med, 1: 505-512, 2012.


Yutaka Kano, David C. Poole, Mizuki Sudo, Toshiro Hirachi, Shinji Miura and Osamu Ezaki. Control of microvascular PO2 kinetics following onset of muscle contractions: Role for AMPK. Am J Physiol Regul Integr Comp Physiol. 2011 Nov;301(5):R1350-7. doi: 10.1152/ajpregu.00294.2011. Epub 2011 Aug 17.PMID: 21849631

Yutaka Kano, Hidetaka Okada, and Yasunori Morioka. What is coefficient of speed endurance to achieve two-hour marathon? J Appl Physiol (1985). 2011 Jan;110(1):289; discussion 294. No abstract available.PMID: 21542173

Tadakatsu Inagaki, Takashi Sonobe, David C. Poole, and Yutaka Kano. Progressive arteriolar vasoconstriction and fatigue during tetanic contractions of rat skeletal muscle are inhibited by α-receptor blockade. J Physiol Sci. 2011 May;61(3):181-9. doi: 10.1007/s12576-011-0134-2. Epub 2011 Feb 11.PMID: 21312014

Miki Tadaishi, Shinji Miura, Yuko Kai,Yutaka Kano, Yuichi Oishi and Osamu Ezaki. Skeletal muscle-specific expression of PGC-1<alpha>-b, an exercise-responsive isoform, improves endurance performance and peak oxygen uptake. PLoS One. 2011;6(12):e28290. doi: 10.1371/journal.pone.0028290. Epub 2011 Dec 8.PMID: 22174785

曽野部 崇,稲垣 薫克,狩野 豊. バイオイメージングによる運動誘発性筋損傷の評価. 日本整形外科学会雑誌(日整会誌 J. Jap. Orthop. Assoc.)85: 415-419, 2011.


Tadakatsu Inagaki, Takashi Sonobe, David C. Poole and Yutaka Kano. Arteriolar vasomotor control and contractile performance during fatiguing tetanic contractions in rat skeletal muscle: role of sympathetic system. Adv Exp Med Biol. 2010;662:309-15. doi: 10.1007/978-1-4419-1241-1_44.PMID: 20204808

Takashi Sonobe, Tadakatsu Inagaki, Mizuki Sudo, David C. Poole, and Yutaka Kano. Gender differences in intracellular Ca2+ accumulation following eccentric contractions of rat skeletal muscle in vivo. Am J Physiol Regul Integr Comp Physiol. 2010 Oct;299(4):R1006-12. doi: 10.1152/ajpregu.00623.2009. Epub 2010 Jul 14.PMID: 20631296


Mizuki Sudo and Yutaka Kano. Myofibers apoptosis occur in the inflammation and regeneration phase following eccentric contractions in rats. J Physiol Sci. 2009 Nov;59(6):405-12. doi: 10.1007/s12576-009-0049-3. Epub 2009 Jul 28.PMID: 19636670


Takashi Sonobe, Tadakatsu Inagaki, David C. Poole, and Yutaka Kano. Intracellular calcium accumulation following eccentric contractions in rat skeletal muscle in vivo: role of stretch-activated channels. Am J Physiol Regul Integr Comp Physiol. 294: R1329-1337, 2008. PubMed

Yutaka Kano, Kazumi Masuda, Hirotaka Furukawa, Mizuki Sudo, Kazuyuki Mito, Kazuyoshi Sakamoto. Histological skeletal muscle damage and surface EMG relationships following eccentric contractions. J Physiol Sci. 2008 Oct;58(5):349-55.


Danielle Padilla, Poul McDonough, Brad J. Behnke, Yutaka Kano, K. Sue Hageman, Timothy I. Musch and David C. Poole. Effects of Type II diabetes on muscle microvascular oxygen pressures. Respir Physiol Neurobiol. 156: 187-195, 2007. PubMed

Kazumi Masuda, Tatsuya Hori, Kai Tanabe, Yutaka Kano, Aki Hirayama, Shoji Nagase. Proanthocyanidin promotes free radical-scavenging activity in muscle tissues and plasma. Appl Physiol Nutr Metab. 32(6):1097-1104, 2007. PubMed


Danielle Padilla, Poul McDonough, Brad J. Behnke, Yutaka Kano, K. Sue Hageman, Timothy I. Musch and David C. Poole. Effects of Type II diabetes on capillary hemodynamics in skeletal muscle. Am J Physiol Heart Circ Physiol. 291: H2439-2444, 2006. PubMed


Yutaka Kano, Danielle Padilla, Brad J. Behnke, K. Sue Hageman, Timothy I. Musch and David C. Poole. Effects of eccentric exercise on microcirculation and microvascular oxygen pressures in rat spinotrapezius muscle. J. Appl. Physiol. 99: 1516-1522, 2005. PubMed


Yutaka Kano, Koji Sampei and Hirokazu Matsudo. Time course of capillary structure changes in rat skeletal muscle following strenuous eccentric exercise. Acta Physiol. Scand. 180: 291-299, 2004. PubMed

Yutaka Kano, Danielle Padilla, K. Sue Hageman, David C. Poole and Timothy I. Musch. Downhill running: a model of exercise hyperemia in the rat spinotrapezius muscle. J. Appl. Physiol. 97:1138-1142, 2004. PubMed


Yutaka Kano, Satoshi Shimegi, Hirotaka Furukawa, Hirokazu Matsudo and Takudo Mizuta. Effects of aging on capillary number and luminal size in rat soleus and plantaris muscles. J Gerontol. A Biol. Sci. Med. Sci. 2002 57: B422-B427. PubMed

Hiroshi Akima, Yutaka Kano, Yoshitaka Enomoto, Masao Ishizu, Morihiko Okada, Yoshie Ohishi, Shigeru Katsuta, Shin-ya Kuno : Muscle function in 164 men and women aged 20 to 84 yr. Med. Sci. Sport Exer. 33: 220-226, 2001.

Yutaka Kano, Satoshi Shimegi, Hideyuki Takahashi, Kazumi Masuda and Shigeru Katsuta : Changes in capillary luminal diameter in rat soleus muscle after hind-limb suspension. Acta Physiol. Scand. 169: 271-276, 2000

Yutaka Kano, Takumi Tanabe, Junichi Nagasawa, Takudo Mizuta. Effect of age on rat aortic responses to acetylcholine and nitric oxide donor (NOC-18). Res. Commun. Mol. Pathol. Pharmacol. 105:173-176, 1999.

Yutaka Kano, Satoshi Shimegi, Kazumi Masuda, Hajime Ohmori and Shigeru Katsuta : Morphological adaptation of capillary network in hypertrophied rat plantaris muscle. Eur. J. Appl. Physiol. 75: 97-101, 1997.

Kazumi Masuda, Yutaka Kano, Shigeru Katsuta : Adaptation of myoglobin in compensatory hypertrophied rat muscle. Acta Physiol. Scand. 160: 327-331, 1997.

Yutaka Kano, Satoshi Shimegi, Kazumi Masuda, Hideki Sakato, Hajime Ohmori and Shigeru Katsuta : Effects of different intensity endurance training on the capillary network in rat skeletal muscle. Int. J. Microcirc. 17: 93-96, 1997.