Department of Engineering Science

Dept.of Engineering Science, Bioscience and Technology Program aims at education and research, from basics to application, on physics, chemistry, and biology, namely the core subjects for the development of quantum devices, materials engineering, and biotechnology that are essential for today's highly-developed communication society.


Profile

Yutaka Kano, Ph.D
Title: Professor
Email: kano @ pc.uec.ac.jp
Phone: +81-424-43-5586
Office: East-6 Bldg. 907
Web:http://www.ecc.es.uec.ac.jp

Research Fields
Applied Physiology: muscle plasticity to exercise, microcirculation, oxygen exchange, cell membrane function.

Summary of Research Activities
The purpose of our research is to examine the mechanisms contributing to the skeletal muscle blood flow, oxygen exchange and cell membrane function on disease muscle conditions such as aging, inactivity and exercise-induced muscle damage. Recently, we have demonstrated that eccentric exercise results in an altered capillary geometry in muscle. To date, however, it is unknown whether microcirculatory function and the capacity to deliver and distribute O2 within the capillary bed is impaired following eccentric exercise. In the present, we study the cell membrane function and oxygen delivery/oxygen utilization sequel to eccentric exercise. The laboratory has a rodent chamber, eccentric exercise equipment, microscopy for bio-imaging, cryostat, phosphorescence quenching equipment and EMG device.

 

Publications

Hiroaki Eshima, Shinji Miura, Nanami Senoo, Koji Hatakeyama, David C. Poole and Yutaka Kano. Improved skeletal muscle Ca2+ regulation in vivo following contractions in mice overexpressing PGC-1α. Am J Physiol Regul Integr Comp Physiol. 2017 Jun 1;312(6):R1017-R1028. doi: 10.1152/ajpregu.00032.2017. Epub 2017 Apr 24.

Aiko Watanabe, David C. Poole and Yutaka Kano. The effects of RSR13 on microvascular PO2 kinetics and muscle contractile performance in the rat arterial ligation model of peripheral arterial disease. Journal of Applied Physiology 

2017

Toru Tomimatsu, Jun Miyazaki, Yutaka Kano, and Takayoshi Kobayashi.
Photothermal imaging of skeletal muscle mitochondria.
Biomedical Optics Express Vol. 8, Issue 6, pp. 2965-2975 (2017)

Mario Wakizaka, Hiroaki Eshima, Yoshinori Tanaka, Hideki Shirakawa, David C. Poole and Yutaka Kano. In vivo Ca2+ dynamics induced by Ca2+ injection in individual rat skeletal muscle fibers. Physiol Rep. 2017 Mar;5(5). pii: e13180. doi: 10.14814/phy2.13180.

Sudo Mizuki, Ando Soichi, and Kano Y. Repeated blood flow restriction induces muscle fiber hypertrophy. Muscle Nerve. 2017 Feb;55(2):274-276. doi: 10.1002/mus.25415.

2016

Yoshinori Tanaka, Tadakatsu Inagaki, DavidC Poole and Yutaka Kano. pH buffering of single rat skeletal muscle fibers in the in vivo environment. Am J Physiol Regul Integr Comp Physiol. 2016 May 15;310(10):R926-33. doi: 10.1152/ajpregu.00501.2015. 

Yoshinori Tanaka, David C. Poole and Yutaka Kano. pH homeostasis in contracting and recovering skeletal muscle: Integrated function of the microcirculation with the interstitium and intramyocyte milieu. Curr Top Med Chem. 2016;16(24):2656-63. Review.

Toshiaki Nakajima, Tomohiro Yasuda, Seiichiro Koide, Tatsuya Yamasoba, Syotaro Obi, Shigeru Toyoda , Yoshiaki Sato, Teruo Inoue and Yutaka Kano.Repetitive restriction of muscle blood flow enhances mTOR signaling pathways in a rat model. Heart Vessels. 2016 Oct;31(10):1685-95. doi: 10.1007/s00380-016-0801-6.

2015

Kohei Yamakoshi, Kazuyoshi Yagishita, Hirotsugu Tsuchimochi, Tadakatsu Inagaki Mikiyasu Shirai, David C Poole and Yutaka Kano Microvascular oxygen partial pressure during hyperbaric oxygen in diabetic rat skeletal muscle.
Am J Physiol Regul Integr Comp Physiol. 2015 Dec 15;309(12):R1512-20. doi: 10.1152/ajpregu.00380.2015. Epub 2015 Oct 14.

Sudo M, Ando S, Poole DC, Kano Y. Blood flow restriction prevents muscle damage but not protein synthesis signaling following eccentric contractions.Physiol Rep. 2015 Jul;3(7). pii: e12449. doi: 10.14814/phy2.12449.

Eshima H, Poole DC, Kano Y. In vivo Ca2+ buffering capacity and microvascular oxygen pressures following muscle contractions in diabetic rat skeletal muscles: fiber-type specific effects. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology. 2015 Jul 15;309(2):R128-37. doi: 10.1152/ajpregu.00044.2015. Epub 2015 May 6.

2014

Eshima H, Poole DC, Kano Y. In vivo calcium regulation in diabetic skeletal muscle. Cell Calcium. 2014 Nov;56(5):381-9. doi: 10.1016/j.ceca.2014.08.008. Epub 2014 Aug 27.

Yutaka Kano, Shinji Miura, Hiroaki Eshima, Osamu Ezaki and David C. Poole.The effects of PGC-1α on control of microvascular PO2 kinetics following onset of muscle contractions. J Appl Physiol (1985). 2014 May 15. pii: jap.00080.2014. [Epub ahead of print]PMID: 24833782



2013
Hiroaki Eshima, Yoshinori Tanaka, Takashi Sonobe, Tadakatsu Inagaki, Toshiaki Nakajima, David C. Poole, and Yutaka Kano: In vivo imaging of intracellular Ca2+ after muscle contractions and direct Ca2+ injection in rat skeletal muscle in diabetes. Am J Physiol Regul Integr Comp Physiol. in press

Yutaka Kano and Kunihiro Sakuma: Effect of aging on the relationship between capillary supply and muscle fiber size. Adv. Aging Res. 2: 37-42, 2013.

Tomonobu Sakurai, Osamu Kashimura, Yutaka Kano, Hideki Ohno, Li Li Ji, Tetsuya Izawa, Thomas M. Best. Role of nitric oxide in muscle regeneration following eccentric muscle contractions in rat skeletal muscle. J Physiol Sci. 2013 Jul;63(4):263-70.

2012
Yutaka Kano,Takashi Sonobe,Tadakatsu Inagaki, Mizuki Sudo and David C Poole. Mechanisms of exercise-induced muscle damage and fatigue: Intracellular calcium accumulation. J .Phys. Fitness Sports Med, 1: 505-512, 2012.

Paul McDonough, Danielle J. Padilla, Yutaka Kano, Timothy I. Musch and David C. Poole, and Brad J. Behnkee. Plasticity of microvascular oxygenation control in rat fast-twitch muscle: Effects of experimental creatine depletion. Respiratory physiology & neurobiology, Respir. Physiol. Neurobiol. 181: 14-20, 2012.

Shunsaku Koga, Yutaka Kano, Thomas Barstow, Leonardo Ferreira, Etsuko Ohmae, Mizuki Sudo, and David Poole: Kinetics of muscle deoxygenation and microvascular PO2 during contractions in rat: Comparison of optical spectroscopy and phosphorescence-quenching techniques. J. Appl. Physiol. 112: 26-32, 2012.

2011
Yutaka Kano, David C. Poole, Mizuki Sudo, Toshiro Hirachi, Shinji Miura, and Osamu Ezaki: Control of microvascular PO2 kinetics following onset of muscle contractions: Role for AMPK. Am. J. Physiol. Regul. Integr. Comp. Physiol. 301: R1350-1357, 2011.

Miki Tadaishi, Shinji Miura, Yuko Kai, Yutaka Kano, Yuichi Oishi and Osamu Ezaki: Skeletal muscle-specific expression of PGC-1<alpha>-b, an exercise-responsive isoform, improves endurance performance and peak oxygen uptake. PLoS ONE 6(12): e28290. doi:10.1371/journal.pone.0028290. 2011.

Tadakatsu Inagaki, Takashi Sonobe, David C. Poole, and Yutaka Kano: Progressive arteriolar vasoconstriction and fatigue during tetanic contractions of rat skeletal muscle are inhibited by a-receptor blockade. J. Physiol. Sci. 61: 181-189, 2011.

Yutaka Kano, Hidetaka Okada, and Yasunori Morioka: What is coefficient of speed endurance to achieve two-hour marathon? J. Appl. Physiol.110: 275-277, 2011.

Takashi Sonobe, Tadakatsu Inagaki and Yutaka Kano: Evaluation of exercise-induced muscle damage by bioimaging technique. J. Jap. Orthop. Assoc. 85: 415-419, 2011. (In Japanese.)

2010
Takashi Sonobe, Tadakatsu Inagaki, Mizuki Sudo, David C. Poole, and Yutaka Kano: Sex differences in intracellular Ca2+ accumulation following eccentric contractions of rat skeletal muscle in vivo. Am. J. Physiol. Regul. Integr. Comp. Physiol. 299: R1006-1012, 2010.

Tadakatsu Inagaki, Takashi Sonobe, David C. Poole and Yutaka Kano: Arteriolar vasomotor control and contractile performance during fatiguing tetanic contractions in rat skeletal muscle: role of sympathetic system. Adv. Exp. Med. Biol. 662: 309-315, 2010.

2009
Mizuki Sudo and Yutaka Kano: Myofibers apoptosis occur in the inflammation and regeneration phase following eccentric contractions in rats. J. Physiol. Sci. 59: 405-412, 2009.

2008
Yutaka Kano, Kazumi Masuda, Hirotaka Furukawa, Mizuki Sudo, Kazuyuki Mito, Kazuyoshi Sakamoto: Histological skeletal muscle damage and surface EMG relationships following eccentric contractions. J. Physiol. Sci. 58: 349-355, 2008.

Takashi Sonobe, Tadakatsu Inagaki, David C. Poole, and Yutaka Kano: Intracellular calcium accumulation following eccentric contractions in rat skeletal muscle in vivo: role of stretch-activated channels. Am. J. Physiol. Regul. Integr. Comp Physiol. 294: R1329-1337, 2008.

2007
Kazumi Masuda, Tatsuya Hori, Kai Tanabe, Yutaka Kano, Aki Hirayama, Shoji Nagase: Proanthocyanidin promotes free radical-scavenging activity in muscle tissues and plasma. Appl. Physio.l Nutr. Metab. 32:1097-1104, 2007.

Danielle Padilla, Paul McDonough, Brad J. Behnke, Yutaka Kano, K. Sue Hageman, Timothy I. Musch and David C. Poole: Effects of Type II diabetes on muscle microvascular oxygen pressures. Respir. Physiol. Neurobiol. 156: 187-195, 2007.

2006
Danielle Padilla, Paul McDonough, Brad J. Behnke, Yutaka Kano, K. Sue Hageman, Timothy I. Musch and David C. Poole: Effects of Type II diabetes on capillary hemodynamics in skeletal muscle. Am. J. Physiol. Heart Circ. Physiol. 291: H2439-2444, 2006.

2005
Yutaka Kano, Danielle Padilla, Brad J. Behnke, K. Sue Hageman, Timothy I. Musch and David C. Poole: Effects of eccentric exercise on microcirculation and microvascular oxygen pressure in rat spinotrapezius muscle. J. Appl. Physiol. 99: 1516-1522, 2005.

2004
Yutaka Kano, Koji Sampei and Hirokazu Matsudo: Time course of capillary structure changes in rat skeletal muscle following strenuous eccentric exercise. Acta Physiol. Scand. 180: 291-299, 2004.

Yutaka Kano, Danielle Padilla, K. Sue Hageman, David C. Poole and Timothy I. Musch: Downhill running: a model of exercise hyperemia in the rat spinotrapezius muscle. J. Appl. Physiol. 97: 1138-1142, 2004.

1994-2003
Kyoko Inaba, Ken-ichi Kato, Masashi Miyamaru, Shinya Kuno, Mitsugi Ogata and Yutaka Kano: A case study on the progress of female sprint performance. Japan J Phys. Educ. 47:463-472, 2002. (In Japanese.)

Yutaka Kano, Satoshi Shimegi, Hirotaka Furukawa, Hirokazu Matsudo and Takudo Mizuta: Effects of aging on capillary number and luminal size in rat soleus and plantaris muscles. J. Gerontol. A Biol. Sci. Med. Sci. 57: B422-B427, 2002.

Hiroshi Akima, Yutaka Kano, Yoshitaka Enomoto, Masao Ishizu, Morihiko Okada, Yoshie Ohishi, Shigeru Katsuta, Shin-ya Kuno: Muscle function in 164 men and women aged 20 to 84 yr. Med. Sci. Sport Exer. 33: 220-226, 2001.

Nobuyuki Watanabe, Yohitaka Enomoto, Keigo Ohyama Byun, Yutaka Kano, Tohifumi Yasui, Ken Miyashita, Shinya Kuno and Shigeru Katsuta: Relationship between hip strength and sprint running performance in sprinters. Japan J Phys. Educ. 45: 520-529, 2000. (In Japanese.)

Yutaka Kano, Satoshi Shimegi, Hideyuki Takahashi, Kazumi Masuda and Shigeru Katsuta: Changes in capillary luminal diameter in rat soleus muscle after hind-limb suspension. Acta Physiol. Scand. 169: 271-276, 2000

Nobuyuki Watanabe, Yohitaka Enomoto, Yutaka Kano, Tohifumi Yasui, Ken Miyashita, Shinya Kuno and Shigeru Katsuta: Gender differences in the relationship between muscle cross-sectional area and sprint running performance in sprinters. Reseach quarterly for athletics. 39: 12-19, 1999. (In Japanese.)

Yutaka Kano, Takumi Tanabe, Junichi Nagasawa, Takudo Mizuta: Effect of age on rat aortic responses to acetylcholine and nitric oxide donor (NOC-18). Res. Commun. Mol. Pathol. Pharmacol. 105: 173-176, 1999.

Tomomi Shiozaki, Yutaka Kano, Shigeyuki Watanabe, Ryuichi Ajisaka, Masao Ishizu, Shigeru Katsuta, Morihiko Okada and Shinya Kuno: Characteristics of muscle oxygenation in elderly men determined by near infraed spectroscopy. Japan J. Phys. Fitness Sports Med. 47: 393-400, 1998. (In Japanese.)

Kazumi Masuda, Yutaka Kano, Hiroshi Nakano, Mitsuharu Inaki and Shigeru Katsuta. Adaptation of myoglobin in rat skeletal muscles to endurance training. Effects of intensity, duration nd period of training. Japan J. Phys. Fitness Sports Med. 47: 561-572, 1998. (In Japanese.)

Yutaka Kano, Satoshi Shimegi, Kazumi Masuda, Hajime Ohmori and Shigeru Katsuta: Morphological adaptation of capillary network in hypertrophied rat plantaris muscle. Eur. J. Appl. Physiol. 75: 97-101, 1997.

Yutaka Kano, Hideyuki Takahashi, Yasunori Morioka, Hiroshi Akima, Ken miyashita, shinya Kuno and Shigeru Kastuta: Relationship between the morphological features of adductor muscles and sprinting performance in sprinters. Japan J Phys. Educ. 41:352-359, 1997.

Kazumi Masuda, Yutaka Kano, Shigeru Katsuta: Adaptation of myoglobin in compensatory hypertrophied rat muscle. Acta Physiol. Scand. 160: 327-331, 1997.

Yutaka Kano, Satoshi Shimegi, Kazumi Masuda, Hideki Sakato, Hajime Ohmori and Shigeru Katsuta: Effects of different intensity endurance training on the capillary network in rat skeletal muscle. Int. J. Microcirc. 17: 93-96, 1997.

Yutaka Kano, Satoshi Shimegi, Kazumi Masuda, Hideki Sakato, Hajime Ohmori and Shigeru Katsuta: Effects of different intensity endurance training on capillary network in rat left ventricular myocardium. Japan J. Phys. Fitness Sports Med. 45: 511-518, 1996. (In Japanese.)

Yutaka Kano, Kiichiro saito, Yoshiki Ohno and Shigeru Katsuta: Effects of endurance training on the morphology of the cardiac myocyte and capillary in the rats. Japan J Phys. Educ. 39: 225-233, 1994. (In Japanese.)